Now showing items 1-20 of 24
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Stress, Homeostasis and Robustness: Molecular and Systems Level Analysis in Yeast
(2012-05-02)Abstract Cells constantly encounter stress due to alterations in the external milieu or internal parameters. However, cells are robust to such changes and maintain homeostasis. Using the yeast Saccharomyces cerevisiae as ... -
Osmoregulation at different stages of the yeast life cycle
(2012-05-02)The ability to adapt to changing and potentially harmful conditions in the surrounding environment is crucial for fitness and survival of all living cells; in particular unicellular organisms, since they are frequently ... -
Design and Synthesis of Potential Aminoacyl-tRNA Synthetase Inhibitors
(2012-04-12)Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes present in all living organisms, their catalytic activity is involved in the translation of the genetic code into functional proteins and they are potential targets ... -
Unravelling the complexity of cold acclimation in plants
(2011-05-19)Many plants respond to low non-freezing temperatures by increasing their freezing tolerance in a process known as cold acclimation. Microarray studies have shown that hundreds of genes are differentially expressed during ... -
Protein Damage Control during Embryonic Stem Cell Differentiation: Role of the Proteasome
(2011-05-11)During the lifespan of organisms ranging from yeast to humans, there is an accumulation of macromolecular damage. However, these organisms produce youthful progeny with low damage levels. This thesis focuses on how this ... -
The Importance of a Fifth Element in Transcription: Transcriptional Discrimination in Escherichia coli
(2011-04-08)Upon growth arrest of the bacterium Escherichia coli, RNA polymerase (Es70) is redirected from transcribing genes encoding the protein synthesizing system (PSS) to those involved in maintenance and stress resistance. The ... -
Post-transcriptional regulation after stress in Schizosaccharomyces pombe
(2011-03-18)Post transcriptional regulation is part of the gene expression control and is important for many cellular processes. It influences how mRNAs are selected for translation, degradation or storage. In this thesis, I describe ... -
Hyperoxia avoidance and aggregation behavior in C. elegans
(2010-10-19)Living in the soil, C. elegans can move in three dimensions in search for food. To navigate, it partly uses oxygen levels as a description of its habitat. Oxygen tension may indicate presence of microbial food and location ... -
From cell populations to single cells - quantitative analysis of osmotic regulation in yeast
(2010-09-06)To date, interdisciplinary research is becoming increasingly popular because it combines the achievements of diverse disciplines, having the potential of providing a completely new angle to pertinent research problems. ... -
General stress proteins: Novel function and signals for induction of stationary phase genes in E.coli
(2010-05-04)Survival during conditions when nutrients become scarce requires adaptation and expression of genes for maintenance in order for the cell to survive. Among the numerous proteins involved in adaptation and regulation under ... -
Oxidative damage and the DNA glycosylase MutYH
(2010-04-15)The DNA glycosylase MutYH is highly conserved throughout evolution, and homologs are found in most eukaryotes and prokaryotes examined. MutYH functions as a base excision repair DNA glycosylase that excises adenines ... -
The Chaperonin Containing TCP-1 : Interactions with the Mammalian Cytoskeleton
(2010-03-31)The eukaryotic Chaperonin Containing TCP-1 (CCT) is a heterooligomeric chaperonin essential for enabling the cytoskeletal proteins actin and tubulin to fold to their native state. The eight CCT subunits are encoded by ... -
The Oxidative and Osmotic Stress Responses of S. cerevisiae
(2009-11-25)The yeast Hog1 is a stress responsive mitogen activated protein kinase (MAPK) similar to mammalian p38 and JNK. Rck2 is a protein kinase downstream of Hog1. The Hog1 pathway was previously implicated exclusively in the ... -
Functional characterisation of the yeast tumour suppressor homologue Sro7p
(2009-10-05)Correct targeting of newly synthesized proteins to appropriate domains of the cell membrane is crucial to cellular architecture, polarity and function, making it no surprise that many proteins of the secretory machinery ... -
Novel Tumor Suppressor Gene Candidates in Experimental Endometrial Carcinoma - From Cytogenetic to Molecular Analysis
(2009-08-28)Endometrial carcinoma (EC) is the most common form of gynecological malignancy, ranking fourth in incidence among tumors diagnosed in women. As is the case with other complex diseases, detailed analyses of the underlying ... -
Microbial ecology in deep granitic groundwater - activity and impact of viruses
(2009-05-13)The deep subsurface environments in granitic rock can be studied and sampled at the Äspö hard rock laboratory (HRL) close to Oskarshamn in Sweden. Here, the groundwater is anaerobic and total counts give numbers of 104 to ... -
Cold Acclimation in oats and other plants: Dissecting low temperature responses using a comparative genomic approach.
(2009-02-27)Cold acclimation protects plants from temperate regions of the world from the deleterious effects of low and freezing temperatures. This is through a series of transcriptional, regulatory and metabolic changes that enable ... -
Gene Expression Patterns in a Rat Model of Human Endometrial Adenocarcinoma
(2008-11-28)Endometrial cancer develops from the endometrium of the uterus and is the most common pelvic malignancy diagnosed in women in the western society. Similar to all cancer diseases, endometrial cancer is a disorder that results ... -
Development and Evaluation of Web Applications for Investigating Candidate Genes in Rat Models of Complex Diseases
(2008-11-21)Many human diseases, such as rheumatoid arthritis and type 2 diabetes mellitus, have a very complex development, depending on both environmental and multiple genetic factors. By crossing inbred rat strains susceptible to ... -
In the quest for a cold tolerant variety - gene expression profile analysis of cold stressed oat and rice
(2008-11-14)Cold acclimation is a process which increases the freezing tolerance of an organism, after exposure to low, non-freezing temperatures. The acclimation ensures that cold tolerant species can endure harsh winter conditions, ...