Visa enkel post

dc.contributor.authorHernebring, Malin
dc.date.accessioned2011-05-11T11:24:46Z
dc.date.available2011-05-11T11:24:46Z
dc.date.issued2011-05-11
dc.identifier.isbn978-91-628-8309-6
dc.identifier.urihttp://hdl.handle.net/2077/25294
dc.description.abstractDuring the lifespan of organisms ranging from yeast to humans, there is an accumulation of macromolecular damage. However, these organisms produce youthful progeny with low damage levels. This thesis focuses on how this is accomplished. I have analyzed whether the levels of oxidatively damaged proteins change in mouse embryonic stem (ES) cells during the initial steps of cell specification (differentiation) from the pluripotent state. The results show that ES cells contain high levels of proteins modified by carbonyls and advanced glycation end products and that the identity of these damaged proteins, including chaperones and proteins of the cytoskeleton, are the same as those of aged tissues. However, early differentiation is accompanied by a dramatic drop in the damage of such proteins, both in cultured ES cells and in the blastocyst in vivo. //Text removed from public version// Moreover, the results support a model in which the restoration of low levels of protein damage at the start of each generation is achieved, in part, by a maintained capacity of the germ line to rid itself from such damage. //Text removed from public version//sv
dc.language.isoengsv
dc.relation.haspartI. Elimination of damaged proteins during differentiation of embryonic stem cells. Hernebring M*, Brolén G*, Aguilaniu H, Semb H & Nyström T. (2006) Proc Natl Acad Sci U S A. 103(20): 7700–7705. * contributed equally to this work ::PMID::16672370sv
dc.relation.haspartII. Identification of Hsc70 as target for AGE modification in senescent human fibroblasts. Unterluggauer H, Micutkova L, Lindner H, Sarg B, Hernebring M, Nyström T & Jansen-Dürr P. (2009) Biogerontology. 10(3): 299-309. ::PMID::19009367sv
dc.relation.haspartIII. The proteasome activator PA28 is required for the removal of damaged proteins during differentiation of mouse embryonic stem cells. Hernebring M, Fredriksson Å, Norrman K, Rivett J, Wiseman J, Semb H & Nyström T. Manuscript.sv
dc.relation.haspartIV. Effects of aging and reproduction on proteostasis in soma and gametes of Drosophila melanogaster. Fredriksson Å, Krogh-Johansson E, Hernebring M, Pettersson E & Thomas Nyström. Manuscript.sv
dc.subjectEmbryonic Stem Cellssv
dc.subjectProteasomesv
dc.subjectProtein Carbonylationsv
dc.subjectAdvanced Glycation End productssv
dc.subjectCell Differentiationsv
dc.subjectOxidative stresssv
dc.subjectAgingsv
dc.subjectRNAisv
dc.titleProtein Damage Control during Embryonic Stem Cell Differentiation: Role of the Proteasomesv
dc.typeText
dc.type.svepDoctoral thesiseng
dc.gup.mailmalin.hernebring@astrazeneca.comsv
dc.type.degreeDoctor of Philosophysv
dc.gup.originGöteborgs universitet. Naturvetenskapliga fakultetensv
dc.gup.departmentDepartment of Cell and Molecular Biology ; Institutionen för cell- och molekylärbiologisv
dc.gup.defenceplaceonsdagen den 1 juni 2011 kl. 10.00 i Carl Kylberg, Institutionen för Cell- och Molekylärbiologi, Medicinaregatan 7B, Göteborgsv
dc.gup.defencedate2011-06-01
dc.gup.dissdb-fakultetMNF


Filer under denna titel

Thumbnail
Thumbnail

Dokumentet tillhör följande samling(ar)

Visa enkel post