| dc.contributor.author | Bandstein, Sara | |
| dc.date.accessioned | 2017-04-12T09:19:30Z | |
| dc.date.available | 2017-04-12T09:19:30Z | |
| dc.date.issued | 2017-04-12 | |
| dc.identifier.uri | http://hdl.handle.net/2077/52216 | |
| dc.description.abstract | Background:
Absolute
uterine
factor
infertility
affects
about
1:500
women
in
fertile
age.
This
can
now
be
cured
by
uterus
transplantation,
a
procedure
that
involves
risky
donor
surgery
and
side
effects
of
immunosuppression.
The
creation
of
a
tissue-‐engineered
uterus/uterine
tissue
using
the
patient’s
own
stem
cells
would
circumvent
these
issues.
Objective:
To
create
a
tissue
engineered
uterine
patch
for
repair
of
a
partially
defect
uterus.
Methods:
Three
different
decellularized
uterine
scaffolds
were
recellularized
in
vitro
with
primary
uterine
cells
and
mesenchymal
stem
cells
(MSCs).
The
patches
were
transplanted
in
vivo
to
investigate
their
tissue
adaptation
and
supporting
capacity
during
pregnancy.
Recellularization
efficiency
and
graft
quality
were
analyzed
morphologically,
immunohistochemically,
and
by
real-‐time
quantitative
polymerase
chain
reaction
(qPCR).
The
location
and
number
of
fetuses
were
documented
during
embryonic
day
16–20.
Results:
Pregnancy
and
fetal
development
were
normal
in
groups
P1
and
P2,
with
fetal
development
over
patched
areas.
Group
P3
showed
significant
reduction
of
fetal
numbers,
and
embryos
were
not
seen
in
the
grafted
area.
No
placentation
was
formed
over
the
patch
areas
in
any
of
the
experiment
groups.
qPCR
and
immunohistochemistry
revealed
uterus-‐
like
tissue
in
the
patches
that
had
been
reconstructed
by
infiltrating
host
cells
after
transplantation.
No
mesenchymal
stem
cells
remained
in
the
grafts.
Conclusions:
Primary
uterine
cells
and
MSCs
can
be
used
to
reconstruct
decellularized
uterine
tissue,
and
the
tissue
engineered
uterine
patch
can
be
used
to
support
pregnancy
in
a
partially
defect
uterus.
The
bioengineered
patches
made
from
triton-‐X100+DMSO-‐
generate
scaffolds
were
supportive
during
pregnancy.
These
protocols
should
be
explored
further
to
develop
suitable
grafting
material
to
repair
a
partially
defect
uteri
and
possibly
to
create
a
whole
bioengineered
uterus
for
a
uterus
transplantation. | sv |
| dc.language.iso | eng | sv |
| dc.subject | Decellularization | sv |
| dc.subject | recellularization | sv |
| dc.subject | tissue engineering | sv |
| dc.subject | uterus | sv |
| dc.title | Tissue engineered uterine tissue supports pregnancy in a rat model | sv |
| dc.title.alternative | Tissue engineered uterine tissue supports pregnancy in a rat model | sv |
| dc.type | Text | |
| dc.setspec.uppsok | Medicine | |
| dc.contributor.department | University of Gothenburg / Institute of Medicine | eng |
| dc.contributor.department | Göteborgs universitet / Institutionen för medicin | swe |
| dc.type.degree | Student essay | |
