| dc.description.abstract | Thyroid hormone (TH), which is secreted by the thyroid gland, exerts significant
effects on the central nervous system (CNS), especially during development, so
that delayed treatment of perinatal hypothyroidism results in severe cognitive
retardation. Continuous postnatal neurogenesis occurs throughout adulthood in
the subventricular zone (SVZ) of the lateral ventricle wall and in the subgranular
zone (SGZ) of the dentate gyrus (DG) of the hippocampal formation. The neural
stem cells in these regions undergo proliferation, migration, differentiation into
functional neurons, and integration into neural networks. These two highly
specialized regions are termed ‘neurogenic niches’, which mainly contain neural
stem cells, endothelial cells, astrocytes, and microglial cells. In the SVZ, there
are also ependymal cells. Many factors, including growth factors and hormones,
have been implicated in the regulation of neurogenesis.
The overall aim of this thesis was to investigate the effects of TH on the different
cell types, including neural stem cells, endothelial cells and astrocytes, in two
neurogenic niches. A postnatal hypothyroidism rat model was set up by adding
PTU to the drinking water from Postnatal Day 1 (P1) to P21. We studied the
acute and long-term effects of postnatal TH deficiency (PTHD) on both
neurogenesis and angiogenesis in the SVZ and dentate gyrus.
In Paper I, we show region-dependent responses to PTHD. The proliferation of
neural stem cells and the total number of granule neurons in the dentate gyrus
were significantly reduced in PTHD rats at P21, whereas no effects on the
SVZ/OB system, as compared with control rats. In addition, after the withdrawal
of PTU at P22, hippocampal neurogenesis increased as a result of increased cell
survival. These changes were paralleled by alterations in the gene expression
2
patterns of growth factors and apoptotic factors, i.e., Fgf2, Ngf, Wnt3a, Vegfa
and Bcl2, at both P21 and P90.
In Paper II, we describe a reduction in angiogenesis at P21 due to PTHD, as
evidenced by reductions in the complexity and density of the microvessels, both
in the neocortex and dentate gyrus. However, these defects were fully recovered
by P90, following PTU withdrawal at P22. In the neocortex, these changes were
paralleled by altered levels of VEGFA and FGF2. Furthermore, we report that
the physiologic plasma concentration of TH promotes proliferation and tubelike
structure formation, and inhibits the death of brain-derived endothelial
(RBE4) cells in vitro.
In Paper III, we investigate the region-specific contribution of astrocytes to the
activities of neural stem cells after T3 (50 nM) treatment. Conditioned medium
(CM) was collected from cultures of SVZ and hippocampal astrocytes after T3
(50 nM) treatment, and was added to the cultures of neural stem cells (NSCs)
from the corresponding brain regions. The CM from T3-treated hippocampal
astrocyte cultures promoted hippocampal NSCs survival by increasing the
proliferation and decreasing the cell death, whereas the CM from T3-treated SVZ
astrocyte cultures did not have similar effects on the activities of the SVZ NSCs.
Interestingly, the migration of neuroblast from both SVZ and hippocampus was
significantly increased after culturing with CM after T3-treatment from
corresponding regions. Furthermore, the astrocytes after T3-treatment from these
two regions displayed different expression patterns for the Bdnf, Noggin, Wnt3a,
Pedf, and Thrb genes, which are implicated in the regulation of neurogenesis.
In summary, this thesis demonstrates the hyper-susceptibility of hippocampal
neurogenesis to PTHD including NSCs and astrocytes, and provides evidence of
a strong linkage between brain angiogenesis and thyroid hormone levels. | en |
